EXOLITUS | Exosome Technologies

Preclinical development

Investigation of biological activity of extracellular vesicles and particles

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Toxicity studies of drugs, food supplements, and functional foods in selected in vitro models

Evaluation of cell tolerance to selected preparation. Possible evaluation parameters are total cellular metabolic activity, viability (determination of LC50, selection of recommended doses for in vivo studies), determination of the cell death pathway, activation of the inflammatory response, oxidative stress, and studies of mitochondrial and glycolytic energy activity. Established models - gastrointestinal tract, respiratory tract, skin, nervous system, mucous membranes, blood vessels, heart and skeletal muscle, stem cells, and immune cells. It is also possible to implement the model according to individual needs.

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Efficacy studies of drugs and medical devices in a selected in vitro model of viral (including COVID-19) or bacterial inflammation in selected tissue

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Studies on the efficacy of anti-inflammatory drugs in detecting markers of immuno-metabolic activity of immune and/or other selected cells primed by viral or bacterial agents. Parameters for evaluation are the production and secretion of inflammatory cytokines and chemokines, metalloproteinases, reactive oxygen and nitrogen compounds, mitochondrial to glycolytic transition, etc. Possible models are the respiratory tract, blood vessels, myocardium, gastrointestinal tract, neuroglial and others, including custom-designed.

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Drug efficacy studies in an in vitro model of myocardial ischemia

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The experimental model tests whether and to what extent preparation protects cardiomyocytes from ischemia-induced death. Possible extension of the study to assess effects on different death pathways (apoptosis, necrosis, ferroptosis, etc.), functionality (contractility), and mitochondrial damage.

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Drug efficacy studies in an in vitro model of cerebral ischemia

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The cell culture model is designed to determine if the tested preparation protects neurons from ischemic death. Optional assessment parameters include neuronal death, number of synapses, synaptic activity and signal quality, levels of the inflammatory activity of astrocytes and microglia, mitochondrial and glycolytic functionality, and levels of inflammatory factors.

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Drug efficacy studies in an in vitro model of neurodegeneration

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The protection of neurons (pure or co-cultured with other brain cells - microglia, astrocytes) from neurotoxic factors is assessed. Additional evaluation parameters can be selected, including neuronal death rate, number of synapses, synaptic activity and signal quality, and astrocyte and microglial inflammatory activity level.

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Evaluation of the effect on mitochondrial function

The impact of the preparation on the efficiency of the mitochondrial respiratory chain and phosphorylation system of the selected tissue, the integrity of the inner and outer membrane, and other parameters characterising mitochondrial health are evaluated.

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Studies on the effect on glycolytic activity

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The effect of the preparation on the glycolysis efficiency of the selected tissue and the capacity of the glycolytic system is evaluated.

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Studies on the induction of an inflammatory response in human immune cells

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The ability of tested substances to cause inflammation in immune cells, such as macrophages, microglia, fibroblasts, astrocytes, etc., is assessed. Inflammation extent is assessed by the production and secretion of cytokines and chemokines, metalloproteinases, active oxygen and nitrogen compounds, transition from mitochondrial-glycolytic energy metabolism, and so on.

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Blood-brain barrier crossing in vitro studies

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Evaluation of the efficiency of in vitro migration of compounds through the blood-brain barrier formed on the membrane of tissue culture insert (consisting of endothelial and astrocyte layers)

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In vitro studies on the passage through the intestinal barrier

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The effect of the preparation on the glycolysis efficiency of the selected tissue and the capacity of the glycolytic system is evaluated.

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In vitro studies on the crossing of the vascular endothelial barrier

Evaluation of the efficiency of the migration of compounds through the vascular barrier formed in vitro on the membrane of the tissue culture insert

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In vitro studies on skin barrier crossing

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Evaluation of the efficiency of the migration of compounds through the skin barrier formed in vitro on the membrane of the tissue culture insert

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In vitro studies on the airway epithelial barrier crossing

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Evaluation of the efficiency of the migration of compounds through the airway epithelial barrier formed in vitro on the tissue culture membrane. A two-layer model simulating the alveoli of the airway epithelium and vascular endothelium is also available.

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Regenerative capacity assessment

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Evaluation of regenerative effects on selected tissue cells by wound healing, total metabolic activity, energy metabolism intensity, and other methods. Possible models - skin (epidermis, mesoderm, hypodermis, full-thickness), respiratory, brain, intestinal, myocardial, skeletal muscle, retinal neuroepithelium, stem cells, immune cells, etc.

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Evaluation of effects on skin maintenance and functionality in a 3D in vitro model

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Investigation of the effect of the tested preparation on skin structure (formation of characteristic layers, their thickness, and shape) and function (production of extracellular matrix proteins) in a 3D skin model cultured in the air-liquid interface.

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Evaluation of anticancer efficacy in 3D tumour models on substrates mimicking natural extracellular matrix.

Evaluation of viability, proliferation, migration, and oncogenic factor production intensity of cancer cells cultured in tumor-mimicking 3D format and close-to-real conditions. Models of glioblastoma, melanoma, intestinal carcinoma, and other tumours are possible.

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Assessment of support for brain function in in vitro models mimicking natural 3D brain structure and functionality

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Assessment of neuronal viability and functionality in 3D in vitro models reproducing the natural cellular composition, structure, and function of the brain

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Assessment of effects on osteogenesis

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Evaluation of effects on osteoblast viability, proliferation and differentiation

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Efficacy studies of neuroregenerative drugs in an in vitro model of Alzheimer's disease

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Sporadic (based on amyloidogenic peptides), familial (based on mutation), or mixed models are available. It is determined whether the preparation protects neuronal synapses, improves their function, reduces the level of inflammation, and improves energy metabolism.

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Evaluation of the degree of oxidation in biological samples, antioxidant efficiency studies

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Determination of the degree of oxidation of proteins, lipids, and nucleic acids in biological samples

Contact Us

EXOLITUS, UAB
Kaunas, Lithuania
Corp. ID 305464858
info@exolitus.com
Don’t hesitate to contact us
+370 664 09669
We're ready to hear your requires
Working Time
Mon-Fri: 9:00 - 17:00 / Closed on weekends
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